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KMID : 0380020040190020118
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 2 p.118 ~ p.124
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Mechanism of Melanogenesis Inhibition by Melanoston Isolated from Yeast
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Lee Seung-Sun
Jeong Ho-Kwon
Oh Cheol
Choe Tae-Boo
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Abstract
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Melanocytes synthesize melanin within discrete organelle termed melanosomes which are transferred to the surrounding keratinocytes and can be produced in varying sizes, numbers and densities. Skin whitening products have become increasingly popular in the past few years. The most successful natural skin whitening agents are: Arbutin, Vitamin C, Kojic acid, Mulberry, which are all tyrosinase inhibitors. In this work, melanoston, a melanogenesis inhibitor isolated from yeast was studied to understand its mechanism of melanogenesis inhibition. It was found that melanoston was not a tyrosinase inhibitor, while when melanoston was applied to the B16 melanoma cell culture media, the intracellular tyrosinase activity was decreased by more than 30％, When B16 melanoma was stimulated with -MSH, cell morphololgy was dramatically changed to have lots of dendrites on the cell membrane surface. On the other hand, B16 was treated with -MSH and melanoston, simultaneously, the change of cell morphology was not so great. This inhibition effect of melanoston was found to be related to the inhibition of intracellular activation and transportation of tyrosinase, which was observed by immunostaining of B16 melanoma using anti-tyrosinase antibody. From these results, melanoston was regarded as an inhibitor to the differentiation of melanoma cells.
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KEYWORD
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Melanogenesis inhibition, melanoma, cell differentiation, UV radiation, tyrosinase
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